Aoi Yuki entered the entertainment industry at the age of four. As a child, she acted in films and dramas. From 1999 until 2002, she made regular appearances on the variety shows Appare Sanma Dai-sensei and Yappari Sanma Dai-sensei which aired on Fuji TV. When she was in fifth grade, she made her debut as a voice Actress.
Until the end of 2006, she was affiliated with the theater company Central. After her admission to college in August 2007, she joined the agency Breath, changing her stage name to Aoi Yūki. She got her first major role in 2008 in Kure-nai as Murasaki Kuhōin. In August of the same year, she moved to the agency Pro-Fit.
When she was 16, she played the seven-year-old Murasaki Kuhōin in Kure-nai. She had two roles in 2008, eight in 2009, and twelve in 2010. She graduated from high school in March 2010, and university in March 2014.
In 2009, she starred in two anime: Anyamaru Tantei Kiruminzuu as Mikogami Riko and Yumeiro Patissiere as Amano Ichigo, respectively. After that, she voiced the protagonists of several of her series, notably Iris from Pokémon: Best Wishes! and Victorique de Blois from Gosick.
In 2011, Yūki voiced the eponymous character of the anime Puella Magi Madoka Magica, Madoka Kaname. In October of the same year, she won the award for best voice actor in the Newtype x Machi Asobi Anime Awards. In 2012, she was awarded the Best Lead Actress Award in the 6th Seiyu Awards.
On March 28, 2012, she released her first solo album Petipa. Her first single, "VisuMania", was released on January 29, 2014, which is used as the ending theme to the anime television series World Conquest Zvezda Plot.
In 2013, Yūki, together with voice Actress Ayana Taketatsu, formed the singing unit Petit Milady. Together, they performed the song "Kagami no Dual-ism" (鏡のデュアル・イズム The Mirror of Dual-ism), which was used as the third opening theme to the 2013 anime television series Yu-Gi-Oh! Zexal II, and in 2014, performed the song "Azurite", which was used as the opening theme to the 2014 anime television series The Pilot's Love Song. In 2015 she played the role of Yuuki Konno in Sword Art Online II, and in 2017 she played the roles of Tanya Degurechaff in Saga of Tanya the Evil and Yoshiko Hanabatake in Aho-Girl.
