Charlene was born in Buffalo New York. She got her start in modeling before making the transition to acting in 2001 with a role on the soap opera, Port Charles. That part on the daytime drama led to guest appearances in several other series, including Joey, Girlfriends, 90210, and as Anna Schick in The Young and the Restless.
From 2005 to 2011, she played Wendy, a waitress that works at the bar, the main setting of the American TV hit How I Met Your Mother. Although at times on the series she was rumored to be Ted's Future wife, Amoia herself didn't believe Wendy would turn out to be "the one". "Wendy had sex with Barney behind the bar on the show and also made out with Ted’s dad, so the likelihood of this character being the mom, I thought, was pretty small anyway, but the fact that fans were rooting for me was awesome!" the Actress has said. On playing Wendy, she has said, "I love playing flawed characters. Wendy is so genuinely sweet and guileless to a point that she doesn’t realize she’s being taken advantage of. Being able to take on these characteristics in a way that is funny and harmless is always so much fun!"
Amoia played Ellie, Kevin (Thomas Ian Nicholas)'s wife, in the fourth American Pie film, released in 2012. She originally auditioned for a different role before landing this role.
She has appeared in such films as Seven Pounds, with Will Smith, and American Reunion. In 2013, she plays Jill in Fat, which premiered at the Toronto International Film Festival.
She played: Janet Conrad, a pushy NATO Photographer who was seeking a career changing "Front Line Photo" in the 2016 movie; Sniper: Special Ops.
In 2017 she appeared on American Horror Story in the episode "Great Again". In season 14 of Grey's Anatomy she appeared as Dr. Kate Lachman.
