Born in London, Cole is the eldest of three siblings. She has a younger sister, Cassandra, and a brother, Dominic. Her mother is a telecentre manager and her father is a driving instructor. Cole trained at the Oxford School of Drama, graduating in 2002.
Cole won the role of Clarissa Payne in What a Girl Wants (2003), featuring Colin Firth and Amanda Bynes, while still at drama school. She graduated early to begin filming. She was then cast as Juliet in a production of Romeo and Juliet at Clwyd Theatr Cymru.
Other roles include a small part in the James Bond film Casino Royale (2006), as the receptionist at the Ocean Club Hotel in Nassau, Bahamas, and Diana Mann in the pilot episode of Cane, Cole's first appearance on American television. She has also appeared as Mel on the ITV sitcom Sold, as Jenny Walker in The Deaths of Ian Stone (2007), and as Charlotte Warren in Miss Pettigrew Lives for a Day (2008).
In 2009, Cole played Dr. Sarah King in Poirot: Appointment with Death, and was cast in the lead role of Maggie Hill, a brilliant surgeon who endures adult-onset schizophrenia, in a pilot for Fox. She also starred in the films Surviving Evil (2009) alongside Natalie Mendoza and Billy Zane, and Doghouse (2009), with Danny Dyer and Billy Murray. Cole stars in the upcoming noir science fiction action thriller film The Scribbler which is directed by Jake West.
In 2012, Cole appeared in a music video for Cheyenne Jackson's Before You.
Since 2015, Cole has portrayed Dr. Paula Agard on a recurring basis in the USA Network series Suits and made a one episode appearance in the NBC series The Blacklist. The same year she also portrayed Mrs. Sprot in the BBC's Partners in Crime series, in two episodes adapting Agatha Christie's N or M?.
In 2016, Cole opened at the Playhouse Theatre as Stevie in the world premiere of Matthew Perry's play The End of Longing, starring Perry, Lloyd Owen and Jennifer Mudge.
