Karpluk began her acting career in Vancouver and between 2000 and 2005, she appeared in more than a dozen television movies and series before landing the role of Kate in Godiva's. Her work during this period included "Voice of Sylan" in the final episode of Dark Angel and the TV movie Family Sins. She was also in a short-lived US television series called Glory Days in 2002. In 2004, she appeared in Legend of Earthsea and played a serial killer in Ripper 2: Letter from Within. She earned a Leo nomination for her first season of Godiva's and a 2006 Gemini nomination for Best Performance by an Actress in a Continuing Leading Dramatic Role. Since Godiva's, Karpluk has continued to work in television and short films. In 2007 she appeared in Bionic Woman, Flash Gordon, and The L Word. She had a leading role in a 2008 television movie, Smokejumpers, directed by John Terlesky.