In 2009 Acaster performed in a show at the Edinburgh Festival Fringe with fellow comedians Josh Widdicombe and Nick Helm. In 2010 he supported Josie Long on tour, and in 2011 he supported Milton Jones on tour.
He appeared on Russell Howard's Good News Extra and was the internet act in Dave's One Night Stand from Bristol. In 2011 he had his first solo show at the Edinburgh Festival, "Amongst Other Things". Acaster appeared in Australia Versus and was co-host in My First Gig on Resonance FM radio. He appeared in Chris Addison's E4 programme Show and Tell in autumn 2011.
In spring 2012 he performed "Amongst Other Things" as solo shows in various venues in the UK. Acaster's 2012 Edinburgh show was called "Prompt". It began its run to positive reviews and enthusiastic audience response. It received a nomination from the Foster's Edinburgh Comedy Awards for best comedy show at the festival in 2012.
Acaster's 2013 Edinburgh show "Lawnmower" was staged at the Pleasance Courtyard. It was nominated for the Foster's Edinburgh Comedy Award 2013 for best comedy show. In September 2013 he made his first appearance on the BBC musical comedy panel show Never Mind the Buzzcocks. He completed his UK tour of "Lawnmower" at a hometown gig in Kettering on 30 November 2013.
Acaster appeared as a panellist on 8 Out of 10 Cats on 17 March and 4 November 2014, and on Spicks and Specks in Australia on 14 May 2014. Acaster has been a guest several times on Mock the Week.
Acaster appeared as a guest on The Dog Ate My Homework in March 2015 and Have I Got News for You in June 2015. On 2 December 2015, he appeared as "Mike" on the BBC Three sitcom Josh. On 7 December 2015, he appeared on Live at the Apollo, performing segments from his show "Represent".
In September 2016 he wrote a pilot episode of a new sitcom "We The Jury" which was broadcast by the BBC.
Acaster appeared on a Would I Lie to You? episode in 2017.
In March 2018 Netflix released Repertoire, consisting of four one hour shows filmed in September 2017.
