Born in Aberdeen, Scotland and raised in Mintlaw, Aberdeenshire, Little began playing football at a young age with her father and brother. She played football for her primary school and local boys teams as a youth. From age 10 to 14, she played at the youth level for Buchan Girls before joining the Hibernian Girls in 2005. At age 13, she attended and played for Mintlaw Academy. During her time with Hibernian Girls, Little would travel about two and half hours each way from Aberdeen to Edinburgh for training. Of her youth, Little said, "Football gave me opportunities from a young age. Having the chance to travel, to play in different environments and to come up against really good players from all over Europe was fantastic and made me more determined to keep playing." Little attended the University of Hertfordshire when she obtained a degree in Sports Studies while playing for Arsenal L.F.C..