She was born and raised in Charleston, South Carolina. Margaret was a member of a local theater company called The Charleston Youth Company which has been serving area youth since 1978.
Margaret Anne starred in Death Cab for Cutie's music video for What Sarah Said from the band's 2005 studio album, Plans. The What Sarah Said video was included in the band's 2006 DVD, Directions: The Plans Video Album, which was nominated for Best Long Form Music Video at the 2007 Grammy Awards.
Florence's first film role was in 2006's My Super Ex-Girlfriend, alongside Uma Thurman and Luke Wilson. In 2009, she played the role of 'Alexis Danella' in Kevin Costner's horror-thriller The New Daughter.
Beginning on July 21, 2008, Margaret Anne starred as 'Luisa' in the revival of the famous Broadway musical The Fantasticks at the Snapple Theater Center (renamed the Jerry Orbach Theater during this run as a credit to that actor's role in the original musical). Margaret Anne's final performance as Luisa took place on December 17, 2008.
Margaret Anne received a Masters in Music Theatre Performance, cum laude, from New York University and a B.A. in Music/Classical Voice, cum laude, from the College of Charleston. She received the Bishop Robert Smith Award, the highest undergraduate honor at the College of Charleston. In 2009, Margaret Anne was honored with the College of Charleston's Young Alumna of the Year Award.
Margaret Anne starred as the female lead in The One, alongside Jon Prescott and Ian Novick. The film was released by Fox Meadow Films in October 2011.
Margaret Anne appeared on the NBC show 30 Rock on February 9, 2012, alongside Tina Fey and Tracy Morgan in the hour-long Valentine's Day episode "Hey Baby, What's Wrong". She appeared in the CBS drama Blue Bloods featuring Tom Selleck in the show's 7th episode, Brothers, on November 5, 2010. She had a recurring, three-episode arc as 'Marlene' during Guiding Light's penultimate season in 2007 and 2008. She also had roles in Law & Order in the 18th season-finale, Excalibur in 2008, White Collar on the USA Network in the 1st season episode Flip of the Coin in 2009, and Showtime's Nurse Jackie in the 2nd season episode Apple Bong in 2010. She also appeared in 2017 in the CMT TV series Sun Records.
