Boone studied acting as a student at Belleview High School, where she graduated in 2001. In 2005, she graduated from Florida State University's School of Theatre with a Bachelor of Fine Arts (BFA) in Acting. Boone studied with Jane Alexander and Edwin Sherin at the Asolo Repertory Theatre and workshopped with Playwright Mark Medoff. Boone credits Jane Alexander with influencing her to continue acting despite expressing an interest in quitting early in her career. Boone is currently pursuing an MBA in Sustainability from Bard College.
Boone starred in the 2007 Los Angeles debut of the Charles L. Mee play Limonade Tous Les Jours and won two LA Weekly Theater Awards for her performance. Boone made her feature film debut in the horror film My Bloody Valentine 3D (2009), followed by a supporting role in Sex and the City 2 in 2010. During 2010 in her year in television, she portrayed Junior Deputy District Attorney Lauren Stanton in the short-lived NBC series Law & Order: LA. That same year, Boone made her directorial debut with the independent film Eggshells for Soil, portions of which were shot in her hometown, The Villages, Florida. She has also appeared in Step Up Revolution (2012), the fourth film in the Step Up series.
Boone would later receive a starring role in the independent drama Leave Me Like You Found Me (2012), for which she won the Gen Art Film Festival Award for Best Actress. She had a recurring role on the CBS police drama series Blue Bloods in 2013, followed by her starring role of FBI agent Elizabeth Keen in the NBC series The Blacklist the same year.. The Blacklist get both critical and public success, including good DVR ratings scores.
In November 2015, Boone's rep confirmed she and Artist Dan Estabrook were expecting their first child together. In January 2016, during an appearance on Live! with Kelly and Michael, Boone revealed she and Estabrook were engaged and expecting a girl. Boone gave birth to daughter Caroline Boone Estabrook, on April 15, 2016.
Following the birth of her daughter, she is increasingly involved in raising awareness of environmental issues. In January 2017, she launches the CarolineAgnes.org Foundation, which helps provide parents with gift-giving options for their children that are environmentally safe.
