Television Producer Norman Lear saw DuBois in a Lanford Wilson play The Hot l Baltimore at the Mark Taper Forum, which led to her being cast in the 1974–1979 CBS comedy series Good Times. DuBois recorded the album Again, Ja'Net DuBois, on her Peanuts and Caviar label, in 1983. DuBois appeared in former Good Times co-star Janet Jackson's 1987 "Control" music video as her mother. In 1992, she co-starred alongside Clifton Davis in And I Still Rise, a play written and directed by Maya Angelou. She co-starred in the films I'm Gonna Git You Sucka (1988) and Charlie's Angels: Full Throttle (2003), and on television in Moesha, The Steve Harvey Show, A Different World, and The Wayans Bros..