Whilst studying, Allsop guest starred on television series such as Neighbours (1995), Blue Heelers (1997) and Halifax f.p. (1998). After graduating in 1998, Allsop returned to Neighbours as a different character for several months.
In 1999 Allsop landed her break through role. She auditioned for Blue Heelers and won the lead role of Constable Jo Parrish. Over the next 5 years she would become one of Blue Heelers' all-time most popular characters, winning a Logie Award for the Most Popular New Female Talent in 2000, and receiving a nomination for a Silver Logie for Most Popular Actress in 2005. She played the role until 2004 when she left to pursue further career options. Her character was killed off in a bombing, this was used as part of a major revamp of the show.
Jane Allsop is married to actor David Serafin, who she met at aged 13 during acting class, but did not start dating him until years later. Serafin had guest roles on Blue Heelers during Jane's time on the show in 2001 and 2003. In May 2006, Allsop had their first son, Indiana Zac Serafin, and in May 2008 had their second son, Jagger Zed Serafin. They welcomed twin girls in December 2016. The couple were married in July 2014.
After Blue Heelers, the next notable role for Allsop was a guest role in the ABC's MDA. Allsop played Lucy Morello, partner of Andrew Morello who have decided to use IVF to become pregnant. In 2005, Allsop appeared in the children's TV show Holly's Heroes for one episode as Abby Dawson. Also during 2005, Allsop guest starred on Last Man Standing.
In one of the first roles after the birth of her first son, Indiana, Allsop portrayed TV Actress and Comedian Noeline Brown in the 2007 tele-movie, The King: The Story of Graham Kennedy. During the same year, Allsop guest starred on Neighbours as Diana Murray, a barrister working with Toadfish Rebecchi (Ryan Moloney) and Rosetta Cammeniti (Natalie Saleeba) on a legal case. Allsop appeared in ten episodes.
Jane Allsop also had recurring roles in Rush and Tangle from 2010. She appeared in Underbelly Files: Tell Them Lucifer was Here and The Slap in 2011. She had a supporting role in House Husbands and the mini series Devil's Dust in 2012. She is now a main cast member of "House Husbands".
