She made her film debut with a supporting role in the 1979 drama Boulevard Nights, which also featured John Fiedler in the cast. She followed this with roles in the films Dead Badge (1995, with Raymond Cruz, Leland Orser, and James B. Sikking) and Black Out (1996, opposite Brad Dourif, Brad Greenquist, and Jeremy Roberts). DuBois also appeared in the television movies about Princess of Monaco, Grace Kelly, opposite Salome Jens and william Schallert, and The Trial of the Incredible Hulk, with John Rhys-Davies.
DuBois previously co-starred opposite Stephen Collins and Elizabeth Lindsey in the television series Tales of the Gold Monkey, which lasted only one season, airing from September 1982 through June 1983. She also had a recurring role on Magnum, P.I. and made guest appearances on such series as Hardcastle & McCormick (starring Brian Keith and Daniel Hugh Kelly), The A-Team, L.A. Law, Silk Stalkings, and Matlock.
In 2000, she co-starred with Scott Bakula and Robert Beltran in the romantic comedy Luminarias. Between 2005 and 2008, DuBois played Sergeant Roberta Hansen in ten McBride television movies opposite John Larroquette.
She portrayed also Dora in the television western Lone Rider (2008, with Mike Starr and Tom Schanley) and had a guest role as Maria Cordero in the Law & Order: Los Angeles episode "Ballona Creek" (2010, with Patrick Fischler, Saxon Trainor, and Tom Virtue).
